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KMID : 0613820190290080871
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Journal of Life Science
2019 Volume.29 No. 8 p.871 ~ p.878
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Regulation of Wnt/β-catenin Signal Transduction in HT-29 Colon Cancer Cells by a Rhododendron brachycarpum Fraction
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Sim Bo-Ram
Nam Young-Sun
Lee Ja-Bok
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Abstract
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The aim of this study was to determine the anticancer activities of an 80% methanol extract and various fractions of Rhododendron brachycarpum (RB). The n-hexane fraction of RB showed the highest inhibitory activity (Inhibit concentration 50%=20.2±1.2 μg/ml) in HT-29 cells. Colony- and sphere- forming abilities were significantly correlated with a decrease in the cell count and size. A TOP/FOP flash reporter assay revealed that the inhibitory activity of the n-hexane fraction of RB (0.22±0.02 fold change) was lower than that of the 80% methanol extract and that of other fractions. The n-hexane and ethyl acetate fractions of RB were predominantly dependent on the expression levels of intracellular β-catenin. Western blotting using p-GSK3β with only the n-hexane fraction of RB was conducted to examine whether these secondary metabolites reduced β-catenin degradation. Intracellular β-catenin regulation resulted in quantitative changes in the nucleus. In summary, these results demonstrate the potential of the n-hexane fraction of RB as a natural anticancer agent.
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KEYWORD
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Anti-cancer, β-catenin, p-GSK3β, Rhododendron brachycarpum, Wnt
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